ABSTRACT
To study the chemical constituents and their biological activities in the rhizomes of Curcuma phaeocaulis, silica gel column chromatography, reverse medium pressure liquid chromatography, preparative thin layer chromatography, and semi-preparative high performance liquid chromatography were used for isolation and purification and modern spectroscopic methods were used to determine the structure of the isolated compound. Moreover, the effect of the compound on the proliferation of HUVECs was determined by the MTT assay. A new elemane-type sesquiterpenoid glycoside was isolated from the n-butanol soluble fraction of 95% ethanolic extract of the rhizomes of Curcuma phaeocaulis. Its structure was identified as (1Z)-2-hydroxy-curzerenone 2-O-β-D-glucoside. It showed no inhibitory effect on the proliferation of HUVECs.
ABSTRACT
Objective: To study the water-soluble chemical constituents from the rhizomes of Atratylodes lancea. Methods: Two sesquiterpenoid glycosides were purified by column chromatography and their structures were determined by spectroscopic analysis. They were also evaluated for anti-inflammatory activity by determining the inhibitory activity on LPS-induced NO and PGE2 generation in RAW 264.7 cell lines. Results: Compound 1 was a new sesquiterpenoid glycoside, named as (1S,4S,5R,7R,10S)-4,11,14-trihydroxy-guai-3-one-11-O-β-D-glucopyranoside, but exhibited no appreciable activity. Compound 2 was atractyloside A and showed weak activity. Conclusion: The hydroxyl group at C-10 of guaiol-type glycoside could be important for anti-inflammatory activity.